Article
Evaluation of the mature grain phytase candidate HvPAPhy_a gene in barley (Hordeum vulgare L.) using CRISPR/Cas9 and TALENs.
Plant molecular biology - 1 Sept 2017
Holme Inger B, Wendt Toni, Gil-Humanes Javier, Deleuran Lise C, Starker Colby G, Voytas Daniel F, Brinch-Pedersen Henrik
Abstract excerpt
In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a specific promoter region of the PAPhy_a gene which contains three overlapping cis-acting regulatory...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
