Article
CRISPR/Cas9-mediated tetra-allelic mutation of the 'Green Revolution' SEMIDWARF-1 (SD-1) gene confers lodging resistance in tef (Eragrostis tef).
Plant biotechnology journal - 1 Sept 2022
Beyene Getu, Chauhan Raj Deepika, Villmer Justin, Husic Nada, Wang Ning, Gebre Endale, Girma Dejene, Chanyalew Solomon, Assefa Kebebew, Tabor Girma, Gehan Malia, McGrone Michael, Yang Meizhu, Lenderts Brian, Schwartz Chris, Gao Huirong, Gordon-Kamm William, Taylor Nigel J, MacKenzie Donald J
Abstract excerpt
Tef is a staple food and a valuable cash crop for millions of people in Ethiopia. Lodging is a major limitation to tef production, and for decades, the development of lodging resistant varieties proved difficult with conventional breeding approaches. We used CRISPR/Cas9 to introduce knockout mutations in the tef orthologue of the rice SEMIDWARF-1 (SD-1) gene to confer semidwarfism and ultimately lodging...
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