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Article

TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice.

Plant biotechnology journal - 1 Jan 2016

Zhang Hui, Gou Feng, Zhang Jinshan, Liu Wenshan, Li Qianqian, Mao Yanfei, Botella José Ramón, Zhu Jian-Kang

Abstract excerpt

CRISPR/Cas9 and TALEN are currently the two systems of choice for genome editing. We have studied the efficiency of the TALEN system in rice as well as the nature and inheritability of TALEN-induced mutations and found important features of this technology. The N287C230 TALEN backbone resulted in low mutation rates (0-6.6%), but truncations in its C-terminal domain dramatically increased efficiency to 25%. In...

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Identifiers

PMID: 25867543
DOI: 10.1111/pbi.12372
PMCID: PMC11388857

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Source receipt: pubmed, retrieved 7 Sept 2026.

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