Article
Detection of CFTR protein in human leukocytes by flow cytometry.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Jul 2014
Johansson Jan, Vezzalini Marzia, Verzè Genny, Caldrer Sara, Bolognin Silvia, Buffelli Mario, Bellisola Giuseppe, Tridello Gloria, Assael Baroukh Maurice, Melotti Paola, Sorio Claudio
Abstract excerpt
Leukocytes have previously been shown to express detectable levels of the protein cystic fibrosis transmembrane conductance regulator (CFTR). This study aims to evaluate the application of flow cytometric (FC) analysis to detect CFTR expression, and changes thereof, in these cells. Aliquots (200 μL) of peripheral whole blood from 12 healthy control volunteers (CTRLs), 12 carriers of a CFTR mutation (CFC), and 40...
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