Article
Quantification of CFTR transcripts.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Ramalho Anabela S, Clarke Luka A, Amaral Margarida D
Abstract excerpt
Quantification and analysis of CFTR transcripts is of crucial importance not only for cystic fibrosis (CF) diagnosis and prognosis, but also in evaluating the efficiency of various therapeutic approaches to CF, including gene therapy. Reverse transcription (RT) followed by quantitative polymerase chain reaction (qPCR) is at present the most sensitive method for transcript abundance measurement. Classical...
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