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Article

Functional consequences of mutating conserved SF2 helicase motifs in the Type III restriction endonuclease EcoP15I translocase domain.

Biochimie - 1 Apr 2013

Mackeldanz Petra, Alves Jürgen, Möncke-Buchner Elisabeth, Wyszomirski Karol H, Krüger Detlev H, Reuter Monika

Abstract excerpt

For efficient DNA hydrolysis, Type III restriction endonuclease EcoP15I interacts with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of two methylation (Mod) subunits and a single restriction (Res) subunit yielding a multifunctional enzyme complex able to methylate or to hydrolyse DNA. Comprehensive sequence alignments, limited proteolysis and mass spectroscopy suggested...

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Identifiers

PMID: 23220200
DOI: 10.1016/j.biochi.2012.11.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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