Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.

Nucleic acids research - 1 Aug 2012

Manakova Elena, Grazulis Saulius, Zaremba Mindaugas, Tamulaitiene Giedre, Golovenko Dmitrij, Siksnys Virginijus

Abstract excerpt

Restriction endonuclease Bse634I recognizes and cleaves the degenerate DNA sequence 5'-R/CCGGY-3' (R stands for A or G; Y for T or C, '/' indicates a cleavage position). Here, we report the crystal structures of the Bse634I R226A mutant complexed with cognate oligoduplexes containing ACCGGT and GCCGGC sites, respectively. In the crystal, all potential H-bond donor and acceptor atoms on the base edges of the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Pairing
Share to Catalytic Domain

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Crystallization

Share to Deoxyribonucleases, Type II Site-Specific

Share to DNA Cleavage

Share to Models, Molecular

Share to Protein Binding

Share to Protein Conformation

Source

Open in PubMed Open full text

Identifiers

PMID: 22495930
DOI: 10.1093/nar/gks300
PMCID: PMC3413111

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.PMID 22495930Read ↗

Isolation of BsoBI restriction endonuclease variants with altered substrate specificity.Semantic proximity Read ↗

Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence.Semantic proximity Read ↗

Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI.Semantic proximity Read ↗

Conversion of the tetrameric restriction endonuclease Bse634I into a dimer: oligomeric structure-stability-function correlations.Semantic proximity Read ↗

Structure, subunit organization and behavior of the asymmetric Type IIT restriction endonuclease BbvCI.Semantic proximity Read ↗

Genetic analysis of the base-specific contacts of BamHI restriction endonuclease.Semantic proximity Read ↗

Protecting recognition sequences on DNA by a cleavage-deficient restriction endonuclease.Semantic proximity Read ↗

Conformational flexibility in T4 endonuclease VII revealed by crystallography: implications for substrate binding and cleavage.Semantic proximity Read ↗

Inference of relationships in the 'twilight zone' of homology using a combination of bioinformatics and site-directed mutagenesis: a case study of restriction endonucleases Bsp6I and PvuII.Semantic proximity Read ↗

Activity and specificity of the bacterial PD-(D/E)XK homing endonuclease I-Ssp6803I.Semantic proximity Read ↗

Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity.Semantic proximity Read ↗

Crystal structures of type II restriction endonuclease EcoO109I and its complex with cognate DNA.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.