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Article

To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells.

PloS one - 1 Jan 2014

Katz Samantha S, Gimble Frederick S, Storici Francesca

Abstract excerpt

Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery. Gene targeting is stimulated by generation of a DNA double-strand break (DSB) at or near the site of correction, but repair of the break via non-homologous end-joining without using the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24558436
DOI: 10.1371/journal.pone.0088840
PMCID: PMC3928301

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SSB

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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