Article
Age-related decrease in TCR repertoire diversity measured with deep and normalized sequence profiling.
Journal of immunology (Baltimore, Md. : 1950) - 15 Mar 2014
Britanova Olga V, Putintseva Ekaterina V, Shugay Mikhail, Merzlyak Ekaterina M, Turchaninova Maria A, Staroverov Dmitriy B, Bolotin Dmitriy A, Lukyanov Sergey, Bogdanova Ekaterina A, Mamedov Ilgar Z, Lebedev Yuriy B, Chudakov Dmitriy M
Abstract excerpt
The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR β repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR β diversity per 10(6) T cells decreases roughly linearly with age, with significant...
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