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Article

Rational optimization of tolC as a powerful dual selectable marker for genome engineering.

Nucleic acids research - 1 Apr 2014

Gregg Christopher J, Lajoie Marc J, Napolitano Michael G, Mosberg Joshua A, Goodman Daniel B, Aach John, Isaacs Farren J, Church George M

Abstract excerpt

Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved in transmembrane transport in E. coli, has been implemented as a selectable/counter-selectable marker, but counter-selection escape frequency using colicin E1 precludes using tolC for inefficient genetic manipulations and/or with...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24452804
DOI: 10.1093/nar/gkt1374
PMCID: PMC3985617

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