Multiplex Generation, Tracking, and Functional Screening of Substitution Mutants Using a CRISPR/Retron System.

Abstract excerpt

We developed a clustered regularly interspaced short palindromic repeats (CRISPR)/retron system for multiplexed generation of substitution mutations by coutilization of a retron system that continuously expresses donor DNA and a CRISPR/Cas9 cassette that induces cleavage at target genomic loci. Our system efficiently introduces substitution mutation in the Escherichia coli genome in a high-throughput manner....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Clustered Regularly Interspaced Short Palindromic Repeats
• Share to Escherichia coli