Article
[Effect of disruption of Pichia pastoris YPS1 gene on viability and production of recombinant proteins].
Genetika - 1 Jun 2013
Sazonova E A, Zobnina A E, Padkina M V
Abstract excerpt
In the present study, we constructed a Pichia pastoris mutant strain PS111 that lacks one member of the yapsin family through disruption of the YPS1 gene coding for aspartic protease yapsin 1. Under normal growth conditions, the PS111 mutant strain did not show detectable growth defects. Unlike the S. cerevisiaeyps1 mutant, the P. pastoris PS111 strain showed no sensitivity when grown in the presence of CaCl2,...
Topics
- Aspartic Acid Endopeptidases
- Calcium Chloride
- Cell Growth Processes
- Fungal Proteins
- Hot Temperature
- Humans
- Hydrogen-Ion Concentration
- Microbial Viability
- Mitochondrial Proteins
- Mutation
- Pichia