Article
Secretion and overproduction of carboxypeptidase Y by a Saccharomyces cerevisiae ssl1 mutant strain.
Bioscience, biotechnology, and biochemistry - 1 Oct 1993
Ichikawa K, Shiba Y, Jigami Y, Serizawa N
Abstract excerpt
Carboxypeptidase Y (CPY; EC 3.4.16.1) is the yeast vacuolar protease. To have CPY secreted and to increase its secretion level, we tried to express the prepro-CPY gene under the control of the inducible GAL10 promoter or constitutive ENO1 promoter on a multicopy plasmid. In the strains KK4, PEP4, and A2-1-1A, carrying the CPY expression plasmid, active CPY was not detected in the culture broth although the CPY...
Topics
- Base Sequence
- Blotting, Western
- Carboxypeptidases
- Cathepsin A
- Culture Media
- Gene Expression Regulation, Fungal
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Saccharomyces cerevisiae