Article
Degradation of HSA-AX15(R13K) when expressed in Pichia pastoris can be reduced via the disruption of YPS1 gene in this yeast.
Journal of biotechnology - 15 Jan 2009
Yao Xue Qin, Zhao Hong Liang, Xue Chong, Zhang Wei, Xiong Xiang Hua, Wang Zhi Wei, Li Xian Yu, Liu Zhi Min
Abstract excerpt
Expression of recombinant protein HSA-AX15(R13K) in Pichia pastoris GS115 strain produced both the intact protein and its two degradation products with molecular weights of around 43kDa and 66.2kDa, respectively. To reduce or avoid the degradation, a modified P. pastoris GS115 stain, in which YPS1 gene was disrupted, was constructed via homologous recombination and used as a host strain for the HSA-AX15(R13K)...
Topics
- Aspartic Acid Endopeptidases
- Blotting, Western
- Cell Proliferation
- Ciliary Neurotrophic Factor
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins
- Humans
- Mutant Proteins
- Mutation