Article
Optimisation of the Schizosaccharomyces pombe urg1 expression system.
PloS one - 1 Jan 2013
Watson Adam T, Daigaku Yasukazu, Mohebi Saed, Etheridge Thomas J, Chahwan Charly, Murray Johanne M, Carr Antony M
Abstract excerpt
The ability to study protein function in vivo often relies on systems that regulate the presence and absence of the protein of interest. Two limitations for previously described transcriptional control systems that are used to regulate protein expression in fission yeast are: the time taken for inducing conditions to initiate transcription and the ability to achieve very low basal transcription in the...
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