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Article

Next-generation sequencing can reveal in vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database.

Tissue antigens - 1 Jan 2014

Holcomb C L, Rastrou M, Williams T C, Goodridge D, Lazaro A M, Tilanus M, Erlich H A

Abstract excerpt

The high-resolution human leukocyte antigen (HLA) genotyping assay that we developed using 454 sequencing and Conexio software uses generic polymerase chain reaction (PCR) primers for DRB exon 2. Occasionally, we observed low abundance DRB amplicon sequences that resulted from in vitro PCR 'crossing over' between DRB1 and DRB3/4/5. These hybrid sequences, revealed by the clonal sequencing property of the 454...

Topics

Alleles
Artifacts
Crossing Over, Genetic
DNA
DNA Primers
Databases, Nucleic Acid
Diagnostic Errors
Exons
Genotype
HLA-DR Antigens
High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Histocompatibility Testing

Humans

Polymerase Chain Reaction

Sequence Analysis, DNA

Source

Identifiers

PMID: 24355006
DOI: 10.1111/tan.12269

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-24355006-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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