Article
The use of modified primer competitors to enhance yields and specificity of HLA class I amplification by polymerase chain reaction (PCR).
Tissue antigens - 1 Feb 2003
Etokebe G E, Wiencke K, Haug T, Spurkland A
Abstract excerpt
Various PCR based techniques have been developed for genomic HLA typing. The fidelity of these techniques is highly dependent upon the specificity of the primers for the given HLA locus. Due to the high degree of homology between HLA class I loci, few primer sites that selectively amplify genes at a given HLA class I locus may be identified. To avoid coamplification of homologous loci, we designed and applied...
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