Concurrent typing of over 4000 samples by long-range PCR amplicon-based NGS and rSSO revealed the need to verify NGS typing for HLA allelic dropouts.

doi:10.1016/j.humimm.2021.04.008

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Article

Concurrent typing of over 4000 samples by long-range PCR amplicon-based NGS and rSSO revealed the need to verify NGS typing for HLA allelic dropouts.

Human immunology - 1 Aug 2021

Kong Denice, Lee Nancy, Dela Cruz Imma Donna, Dames Charlyn, Maruthamuthu Stalinraja, Golden Todd, Rajalingam Raja

Abstract excerpt

Hematopoietic stem cell transplantation (HSCT) from HLA-matched donors significantly decreases the risks of graft-rejection and graft-versus-host disease. Long-range PCR- amplicon-based next-generation sequencing (NGS) is increasingly used as a standalone method in clinical laboratories to determ...

Topics

Alleles
HLA Antigens
Haplotypes
Hematopoietic Stem Cell Transplantation
High-Throughput Nucleotide Sequencing
Histocompatibility Testing
Humans
Oligonucleotides
Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 33980471
DOI: 10.1016/j.humimm.2021.04.008

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-33980471-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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