Article
[Analysis for 2 samples with HLA-DQB1 allele dropout at exon 2 in sequence-based typing].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Aug 2014
Gao Suqing, Wang Daming, Xu Yunping, Deng Zhihui
Abstract excerpt
OBJECTIVE: To explore the reason for HLA-DQB1 allele dropout during routine sequence-based typing(SBT) in order to improve the accuracy of typing. METHODS: Two thousand samples derived from HLA high-resolution typing laboratory were typed for HLA-DQB1 locus using an AlleleSEQR HLA-DQB1 SBT kit. Non-conclusive results and "abnormal" sequencing samples were retyped using a LABType rSSO HD HLA-DQB1 kit and further...
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