Article
Genotype-related fingerprints from HLA-DPB1 exon 2 low-stringency PCR.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Dec 1996
Porfirio B, Chiarelli I, Rombolà G, Mattiuz P L
Abstract excerpt
Techniques based on the formation of heteroduplexes between relevant heterologous amplified HLA loci have proved to be simple and cost-effective screening methods for the detection of DNA sequence diversity. However, the banding patterns produced may not be as complex as required. We used the ori...
Topics
- Bone Marrow Transplantation
- DNA Fingerprinting
- Exons
- Genotype
- HLA-DP Antigens
- HLA-DP beta-Chains
- Humans
- Polymerase Chain Reaction
- Reproducibility of Results
- Sensitivity and Specificity