Article
Efficient detection of the V600E mutation of the BRAF gene in papillary thyroid carcinoma using multiplex allele-specific polymerase chain reaction combined with denaturing high-performance liquid chromatography.
Genetics and molecular research : GMR - 24 Oct 2013
Gong R X, Gong Y P, Yang J, Wei T, Li-Ling J, Zhu J Q
Abstract excerpt
Mutations in the V-raf murine sarcoma viral oncogene homolog B1 gene (BRAF) play an important role in the pathogenesis of papillary thyroid cancer (PTC). In this study, a BRAF V600E mutation was detected in formalin-fixed and paraffin-embedded PTC samples using multiplex allele-specific polymerase chain reaction and denaturing high-performance liquid chromatography. The sensitivity of the assays was validated in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.