Article
Detection of BRAF mutation in thyroid papillary carcinomas by mutant allele-specific PCR amplification (MASA).
European journal of endocrinology - 1 Feb 2006
Sapio Maria Rosaria, Posca Daniela, Troncone Giancarlo, Pettinato Guido, Palombini Lucio, Rossi Guido, Fenzi Gianfranco, Vitale Mario
Abstract excerpt
OBJECTIVE: The somatic point mutation in the BRAF gene, which results in a valine-to-glutamate substitution at residue 600 (BRAF(V600E)), is an ideal hallmark of papillary thyroid carcinoma (PTC). However, its prevalence is varyingly reported in different studies, and its expression in the follicular variant PTC is controversial, reducing its potential usefulness as diagnostic marker. DESIGN AND METHODS: We...
Topics
- Adult
- Alleles
- Carcinoma, Papillary
- DNA, Neoplasm
- Exons
- Female
- Humans
- Male
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Proto-Oncogene Proteins B-raf
- Retrospective Studies