Article
Fluorescence-based temperature control for polymerase chain reaction.
Analytical biochemistry - 1 Mar 2014
Sanford Lindsay N, Wittwer Carl T
Abstract excerpt
The ability to accurately monitor solution temperature is important for the polymerase chain reaction (PCR). Robust amplification during PCR is contingent on the solution reaching denaturation and annealing temperatures. By correlating temperature to the fluorescence of a passive dye, noninvasive monitoring of solution temperatures is possible. The temperature sensitivity of 22 fluorescent dyes was assessed....
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