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Article

Spatial DNA melting analysis for genotyping and variant scanning.

Analytical chemistry - 15 Mar 2009

Crews Niel, Wittwer Carl T, Montgomery Jesse, Pryor Rob, Gale Bruce

Abstract excerpt

A continuous-flow, temperature gradient microfluidic device was used to demonstrate spatial DNA melting analysis with the resolution and reproducibility necessary for clinical SNP scanning and genotyping of human genomic DNA. With a steady-state temperature gradient of 20-30 degrees C across a sample, melting curves were constructed from a single fluorescence data acquisition. This technique was used to scan for...

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Identifiers

PMID: 19228012
DOI: 10.1021/ac801495w

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VKORC1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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