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Article

Rationally Designed Universal Melting Probes (Uni-Melt) for Multiplex Genotyping.

Analytical chemistry - 10 Dec 2024

Zhuang Xinyu, Zhu Yuxuan, Hsing I-Ming

Abstract excerpt

Polymerase chain reaction (PCR) with probe-based melting curve techniques has been widely used in multiplex genotyping for early diagnosis of genetic diseases and common cancers. However, traditional designs face limitations due to the high modification cost of sensing probes and complex background fluorescence signals. Herein, we introduce the Universal Melting Probes (Uni-Melt) system, a closed-tube PCR assay...

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Identifiers

PMID: 39600140
DOI: 10.1021/acs.analchem.4c03050

Genes

SLC25A13

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