Article
Multiplex fluorescence melting curve analysis for mutation detection with dual-labeled, self-quenched probes.
PloS one - 28 Apr 2011
Huang Qiuying, Liu Zanzan, Liao Yiqun, Chen Xiaoyun, Zhang Yi, Li Qingge
Abstract excerpt
Probe-based fluorescence melting curve analysis (FMCA) is a powerful tool for mutation detection based on melting temperature generated by thermal denaturation of the probe-target hybrid. Nevertheless, the color multiplexing, probe design, and cross-platform compatibility remain to be limited by using existing probe chemistries. We hereby explored two dual-labeled, self-quenched probes, TaqMan and shared-stem...
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