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Article

Influencing factors of dsDNA dye (high-resolution) melting curves and improved genotype call based on thermodynamic considerations.

Analytical biochemistry - 1 Feb 2009

Schütz Ekkehard, von Ahsen Nicolas

Abstract excerpt

High-resolution melting of dsDNA using suitable dyes is a simple and cost-effective alternative for mutation scanning. Analytical variation can result from salt and template concentration (C(T)). To overcome this problem the van't Hoff transition enthalpy DeltaH(vH) from dsDNA melting curves was...

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PMID: 19027705
DOI: 10.1016/j.ab.2008.10.045

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19027705-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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