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Article

Semi-rational approach for converting a GH1 β-glycosidase into a β-transglycosidase.

Protein engineering, design & selection : PEDS - 1 Jan 2014

Teze David, Hendrickx Johann, Czjzek Mirjam, Ropartz David, Sanejouand Yves-Henri, Tran Vinh, Tellier Charles, Dion Michel

Abstract excerpt

A large number of retaining glycosidases catalyze both hydrolysis and transglycosylation reactions, but little is known about what determines the balance between these two activities (transglycosylation/hydrolysis ratio). We previously obtained by directed evolution the mutants F401S and N282T of Thermus thermophilus β-glycosidase (Ttβ-gly, glycoside hydrolase family 1 (GH1)), which display a higher...

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Identifiers

PMID: 24287187
DOI: 10.1093/protein/gzt057

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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