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Article

Probing the determinants of the transglycosylation/hydrolysis partition in a retaining α-l-arabinofuranosidase.

New biotechnology - 25 May 2021

Zhao Jiao, Tandrup Tobias, Bissaro Bastien, Barbe Sophie, Poulsen Jens-Christian N, André Isabelle, Dumon Claire, Lo Leggio Leila, O'Donohue Michael J, Fauré Régis

Abstract excerpt

The use of retaining glycoside hydrolases as synthetic tools for glycochemistry is highly topical and the focus of considerable research. However, due to the incomplete identification of the molecular determinants of the transglycosylation/hydrolysis partition (t/h), rational engineering of retaining glycoside hydrolases to create transglycosylases remains challenging. Therefore, to understand better the factors...

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PMID: 33524585
DOI: 10.1016/j.nbt.2021.01.008

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