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Article

Rational design of a GH1 beta-glycosidase to prevent self-condensation during the transglycosylation reaction.

Protein engineering, design & selection : PEDS - 1 Jan 2010

Tran Vinh, Hoffmann Lionel, Rabiller Claude, Tellier Charles, Dion Michel

Abstract excerpt

Mutant N282T of a thermostable beta-glycosidase from GH1 family (TtbetaGly) presenting a high transglycosidase activity was previously obtained by directed evolution. However, it displays a self-condensation activity with the donor 2-nitrophenyl-beta-d-galactopyranoside (oNPGal) which competes with the condensation reaction and entails undesirable effects. In order to prevent this reaction, we rationally modified...

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Source

Identifiers

PMID: 19926593
DOI: 10.1093/protein/gzp068

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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