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Article

Converting a {beta}-glycosidase into a {beta}-transglycosidase by directed evolution.

The Journal of biological chemistry - 4 Nov 2005

Feng Hui-Yong, Drone Jullien, Hoffmann Lionel, Tran Vinh, Tellier Charles, Rabiller Claude, Dion Michel

Abstract excerpt

Directed evolution was applied to the beta-glycosidase of Thermus thermophilus in order to increase its ability to synthesize oligosaccharide by transglycosylation. Wild-type enzyme was able to transfer the glycosyl residue with a yield of 50% by self-condensation and of about 8% by transglycosylation on disaccharides without nitrophenyl at their reducing end. By using a simple screening procedure, we could...

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Identifiers

PMID: 16085651
DOI: 10.1074/jbc.M502873200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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