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Article

ENU-induced mutagenesis in grass carp (Ctenopharyngodon idellus) by treating mature sperm.

PloS one - 1 Jan 2011

Jiang Xia-Yun, Sun Cheng-Fei, Zhang Quan-Gen, Zou Shu-Ming

Abstract excerpt

N-ethyl-N-nitrosourea (ENU) mutagenesis is a useful approach for genetic improvement of plants, as well as for inducing functional mutants in animal models including mice and zebrafish. In the present study, mature sperm of grass carp (Ctenopharyngodon idellus) were treated with a range of ENU concentrations for 45 min, and then wild-type eggs were fertilized. The results indicated that the proportion of embryos...

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Identifiers

PMID: 22022617
DOI: 10.1371/journal.pone.0026475
PMCID: PMC3195716

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