Article
High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library.
BMC molecular biology - 15 Sept 2010
Ishikawa Tomoko, Kamei Yasuhiro, Otozai Shinji, Kim Jinhyong, Sato Ayuko, Kuwahara Yoshikazu, Tanaka Minoru, Deguchi Tomonori, Inohara Hidenori, Tsujimura Tohru, Todo Takeshi
Abstract excerpt
BACKGROUND: During the last two decades, DNA sequencing has led to the identification of numerous genes in key species; however, in most cases, their functions are still unknown. In this situation, reverse genetics is the most suitable method to assign function to a gene. TILLING (Targeting Induc...
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