Article
Characterization of the imprinting signature of mouse embryo fibroblasts by RNA deep sequencing.
Nucleic acids research - 1 Feb 2014
Tran Diana A, Bai Angela Y, Singh Purnima, Wu Xiwei, Szabó Piroska E
Abstract excerpt
Mouse embryo fibroblasts (MEFs) are convenient sources for biochemical studies when cell number in mouse embryos is limiting. To derive the imprinting signature of MEFs and potentially detect novel imprinted genes we performed strand- and allele-specific RNA deep sequencing. We used sequenom allelotyping in embryo and adult organs to verify parental allele-specific expression. Thirty-two known ubiquitously...
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