Article
Production of Sry knockout mouse using TALEN via oocyte injection.
Scientific reports - 5 Nov 2013
Kato Tomoko, Miyata Kohei, Sonobe Miku, Yamashita Satoshi, Tamano Moe, Miura Kento, Kanai Yoshiakira, Miyamoto Shingo, Sakuma Tetsushi, Yamamoto Takashi, Inui Masafumi, Kikusui Takefumi, Asahara Hiroshi, Takada Shuji
Abstract excerpt
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the Y chromosome, using microinjection of TALEN RNA into pronuclear stage oocytes. As expected, the knockout mouse had female external and internal genitalia, a female...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.