Article
High efficiency recombineering in lactic acid bacteria.
Nucleic acids research - 1 May 2012
van Pijkeren Jan-Peter, Britton Robert A
Abstract excerpt
The ability to efficiently generate targeted point mutations in the chromosome without the need for antibiotics, or other means of selection, is a powerful strategy for genome engineering. Although oligonucleotide-mediated recombineering (ssDNA recombineering) has been utilized in Escherichia coli for over a decade, the successful adaptation of ssDNA recombineering to gram-positive bacteria has not been reported....
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