Article
Precise marker excision system using an animal-derived piggyBac transposon in plants.
The Plant journal : for cell and molecular biology - 1 Feb 2014
Nishizawa-Yokoi Ayako, Endo Masaki, Osakabe Keishi, Saika Hiroaki, Toki Seiichi
Abstract excerpt
Accurate and effective positive marker excision is indispensable for the introduction of desired mutations into the plant genome via gene targeting (GT) using a positive/negative counter selection system. In mammals, the moth-derived piggyBac transposon system has been exploited successfully to eliminate a selectable marker from a GT locus without leaving a footprint. Here, we present evidence that the piggyBac...
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