Article
Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
Development (Cambridge, England) - 1 Feb 2014
Guo Xiaogang, Zhang Tiejun, Hu Zheng, Zhang Yanqi, Shi Zhaoying, Wang Qinhu, Cui Yan, Wang Fengqin, Zhao Hui, Chen Yonglong
Abstract excerpt
For the emerging amphibian genetic model Xenopus tropicalis targeted gene disruption is dependent on zinc-finger nucleases (ZFNs) or transcription activator-like effector nucleases (TALENs), which require either complex design and selection or laborious construction. Thus, easy and efficient geno...
Topics
- Animals
- Base Sequence
- CRISPR-Cas Systems
- DNA, Intergenic
- Embryo, Nonmammalian
- Gene Dosage
- Gene Targeting
- Genetic Engineering
- Genetic Loci
- Genome
- Germ Cells
- Inheritance Patterns
- Molecular Sequence Data
- Mutation
- Phenotype
- RNA
- RNA, Messenger
- Xenopus