Article
A remote palm domain residue of RB69 DNA polymerase is critical for enzyme activity and influences the conformation of the active site.
PloS one - 1 Jan 2013
Jacewicz Agata, Trzemecka Anna, Guja Kip E, Plochocka Danuta, Yakubovskaya Elena, Bebenek Anna, Garcia-Diaz Miguel
Abstract excerpt
Non-conserved amino acids that are far removed from the active site can sometimes have an unexpected effect on enzyme catalysis. We have investigated the effects of alanine replacement of residues distant from the active site of the replicative RB69 DNA polymerase, and identified a substitution in a weakly conserved palm residue (D714A), that renders the enzyme incapable of sustaining phage replication in vivo....
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