Article
Perturbation of the Conformational Dynamics of an Active-Site Loop Alters Enzyme Activity.
Structure (London, England : 1993) - 1 Dec 2015
Gagné Donald, French Rachel L, Narayanan Chitra, Simonović Miljan, Agarwal Pratul K, Doucet Nicolas
Abstract excerpt
The role of internal dynamics in enzyme function is highly debated. Specifically, how small changes in structure far away from the reaction site alter protein dynamics and overall enzyme mechanisms is of wide interest in protein engineering. Using RNase A as a model, we demonstrate that elimination of a single methyl group located >10 Å away from the reaction site significantly alters conformational integrity and...
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