Article
Enhancement of protein translocation across the membrane by specific mutations in the hydrophobic region of the signal peptide.
Journal of bacteriology - 1 Mar 1990
Goldstein J, Lehnhardt S, Inouye M
Abstract excerpt
The hydrophobic region of the signal peptide of the OmpA protein of the Escherichia coli outer membrane was extensively altered in its hydrophobicity and predicted secondary structure by site-specific mutagenesis. The mutated signal peptides were fused to nuclease A from Staphylococcus aureus, an...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Base Sequence
- Cell Membrane
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Processing, Post-Translational
- Protein Sorting Signals