Article
In vivo effect of asparagine in the hydrophobic region of the signal sequence.
The Journal of biological chemistry - 5 Aug 1991
Goldstein J, Lehnhardt S, Inouye M
Abstract excerpt
On the basis of the biophysical studies on the synthetic mutant (Ile-8----Asn) OmpA signal peptide in the preceding paper (Hoyt, D. C., and Gierasch, L.M. (1991) J. Biol. Chem. 266, 14406-14412), the in vivo effects of the same mutation were examined by fusing the mutant OmpA signal sequence to Staphylococcus aureus nuclease or TEM beta-lactamase. The mutation in which the isoleucine residue at position 8 of the...
Topics
- Amino Acid Sequence
- Asparaginase
- Bacterial Outer Membrane Proteins
- Base Sequence
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Lipid Bilayers
- Molecular Sequence Data
- Mutation
- Precipitin Tests