Article
Effects of signal peptide mutations on processing of Bacillus stearothermophilus alpha-amylase in Escherichia coli.
Microbiology (Reading, England) - 1 Mar 1995
Suominen I, Meyer P, Tilgmann C, Glumoff T, Glumoff V, Käpylä J, Mäntsälä P
Abstract excerpt
Bacillus stearothermophilus alpha-amylase has a signal peptide typical for proteins exported by Gram-positive bacteria. There is only one signal peptidase processing site when the protein is exported from the original host, but when it is exported by Escherichia coli, two alternative sites are utilized. Site-directed mutagenesis was used to study the processing in E. coli. Processing sites for 13 B....
Topics
- Amino Acid Sequence
- Binding Sites
- Chromosome Mapping
- Escherichia coli
- Genes, Bacterial
- Geobacillus stearothermophilus
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Processing, Post-Translational