Article
Mutations in 16S rRNA in Escherichia coli at methyl-modified sites: G966, C967, and G1207.
Nucleic acids research - 11 Aug 1991
Jemiolo D K, Taurence J S, Giese S
Abstract excerpt
Mutations were constructed at three sites in 16S rRNA in E. coli by oligonucleotide-directed mutagenesis, and cloned into the rrnB operon on either pKK3535 or pNO2680. The mutated bases, G966, C967, and G1207, are located in the 3' major domain of 16S rRNA and are sites post-transcriptionally modified by methylation. We constructed a deletion mutation at C967 (delta 967) and three substitution mutations at each...
Topics
- Base Sequence
- Chromosome Deletion
- Escherichia coli
- Genes, Dominant
- Genes, Lethal
- Methylation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Phenotype
- RNA Processing, Post-Transcriptional