Article
Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes.
Proceedings of the National Academy of Sciences of the United States of America - 1 May 1990
Santer M, Bennett-Guerrero E, Byahatti S, Czarnecki S, O'Connell D, Meyer M, Khoury J, Cheng X, Schwartz I, McLaughlin J
Abstract excerpt
To investigate the function of base 792 of 16S rRNA in 30S ribosomes of Escherichia coli, the wild-type (adenine) residue was changed to guanine, cytosine, or uracil by oligonucleotide-directed mutagenesis. Each base change conferred a unique phenotype on the cells. Cells containing plasmid pKK3535 with G792 or T792 showed no difference in generation time in LB broth containing ampicillin, whereas cells with C792...
Topics
- Base Sequence
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- RNA, Ribosomal
- RNA, Ribosomal, 16S