Article
A mutation at the universally conserved position 529 in Escherichia coli 16S rRNA creates a functional but highly error prone ribosome.
RNA (New York, N.Y.) - 1 Mar 1995
Santer U V, Cekleniak J, Kansil S, Santer M, O'Connor M, Dahlberg A E
Abstract excerpt
A base substitution of G to U was constructed at position 529 in Escherichia coli 16S rRNA. The U529 mutant ribosomes were functional and present on polysomes but were highly error prone and caused a progressive loss of cell viability. They displayed elevated levels of readthrough of stop codons and frameshifting, and an increase in thermal sensitivity of beta-galactosidase, suggestive of missense errors. These...
Topics
- Base Sequence
- Conserved Sequence
- Escherichia coli
- Hot Temperature
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Polyribosomes
- Protein Biosynthesis