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Article

PRP18, a protein required for the second reaction in pre-mRNA splicing.

Molecular and cellular biology - 1 Jan 1990

Vijayraghavan U, Abelson J

Abstract excerpt

We have investigated the role of a novel temperature-sensitive splicing mutation, prp18. We had previously demonstrated that an accumulation of the lariat intermediate of splicing occurred at the restrictive temperature in vivo. We have now used the yeast in vitro splicing system to show that extracts from this mutant strain are heat labile for the second reaction of splicing. The heat inactivation of prp18...

Topics

Cloning, Molecular
Fungal Proteins
Genes
Genes, Fungal
Genetic Complementation Test
Hot Temperature
Macromolecular Substances
Mutation
RNA Splicing
RNA, Messenger
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae

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Identifiers

PMID: 2403639
DOI: 10.1128/mcb.10.1.324-332.1990
PMCID: PMC360747

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2403639-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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