Article
Reversion of autonomously replicating sequence mutations in Saccharomyces cerevisiae: creation of a eucaryotic replication origin within procaryotic vector DNA.
Molecular and cellular biology - 1 Jan 1990
Kipling D, Kearsey S E
Abstract excerpt
To investigate how a defective replicon might acquire replication competence, we have studied the reversion of autonomously replicating sequence (ARS) mutations. By mutagenesis of a Saccharomyces cerevisiae plasmid lacking a functional origin of replication, we have obtained a series of cis-actin...
Topics
- Base Sequence
- DNA Replication
- DNA, Fungal
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Regulatory Sequences, Nucleic Acid
- Saccharomyces cerevisiae
- Structure-Activity Relationship