Article
Mutational analysis of the consensus sequence of a replication origin from yeast chromosome III.
Molecular and cellular biology - 1 Aug 1990
Van Houten J V, Newlon C S
Abstract excerpt
Yeast autonomously replicating sequence (ARS) elements contain an 11-base-pair core consensus sequence (5'-[A/T]TTTAT[A/G]TTT[A/T]-3') that is required for function. The contribution of each position within this sequence to ARS activity was tested by creating all possible single-base mutations within the core consensus sequence of ARS307 (formerly called the C2G1 ARS) and testing their effects on high-frequency...
Topics
- Base Sequence
- Chromosome Deletion
- Chromosome Mapping
- Chromosomes, Fungal
- DNA Replication
- DNA, Fungal
- Escherichia coli
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Phenotype