Article
Cloning of an autonomously replicating sequence (ars) from the Bacillus subtilis chromosome.
Molecular microbiology - 1 Feb 1992
Moriya S, Atlung T, Hansen F G, Yoshikawa H, Ogasawara N
Abstract excerpt
Cloning of an autonomously replicating sequence (ars) from the origin region of Bacillus subtilis was previously unsuccessful because of the strong incompatibility exerted by sequences located within the oriC region. Using an ars searching vector which would be selective for drug resistance even at one copy per cell, and by cloning large fragments covering as much as possible of the oriC region, we have succeeded...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Replication
- DNA, Bacterial
- Kinetics
- Molecular Sequence Data
- Mutation
- Plasmids